Decreased tubular morphogenesis of IBE cells overexpressing Shb in response to endostatin.

IBE/Shb cells were grown in collagen as in Figure 2 for 48 hours in the absence of FGF-2, presence of 5 ng/mL FGF-2, or presence of FGF-2 and 1 μg/mL endostatin, as indicated. Scoring of the endostatin-induced decrease in tubular morphogenesis (defined as tubular structures with a diameter of 1 cell and a length of more than 7 cells) led to the estimation that endostatin diminished the number of tubular structures by 54% ± 1.9% (n = 3, P < .01) in the IBE/Shb cells.