Fig. 5.
![Fig. 5. Identification of a Ca++-dependent and Ca++-independent pathway of [3H]AA release. / Cells were preincubated for 5 minutes at 37°C in the presence (○) or absence (•) of 1 mmol/L Ca++ and 1 mmol/L Mg++. Cells were then stimulated with the indicated concentration of LTB4. Data represent the mean ± SEM of 3 to 4 independent experiments and show (A) the maximal increase in [Ca++]c and (B) the release of [3H]AA release at 60 seconds, where an asterisk indicates that the stimulation of the LTB4-induced Ca++-independent release was significant (P < .05) compared to controls.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/11/10.1182_blood.v95.11.3541/6/bloo01111005x.jpeg?Expires=1722781990&Signature=WD7~lmArsJg86H3mW1pT4gPB02M6sk2njvByYEeOw6alj~QQzsQWqM21kbLGs1JttYzzn-8pNPHD2I78u7GMZM9U2rTe7QYh4lWCdpr3taGs6l9AQB4v8inUrrK2yPjOT87UrTADPDieChX9myulF-AsRfoGQ3tYOQu24LmRFFvtylWZR6G7w~a2sPBsBWO4apFr6kAWv4mfGrNV8Nab-nnQcXhYwp719EHG6i9LHU6zPquX8D9mzT-WrOI31VY-T~1HdicaZn8Fknofq71aRqQlGrp3Fmyc4YM3P1rqMk8G66SJGta~VfHG9zlKPShXYw0N7fqjjCB~zemQkhZcdQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Identification of a Ca++-dependent and Ca++-independent pathway of [3H]AA release.
Cells were preincubated for 5 minutes at 37°C in the presence (○) or absence (•) of 1 mmol/L Ca++ and 1 mmol/L Mg++. Cells were then stimulated with the indicated concentration of LTB4. Data represent the mean ± SEM of 3 to 4 independent experiments and show (A) the maximal increase in [Ca++]c and (B) the release of [3H]AA release at 60 seconds, where an asterisk indicates that the stimulation of the LTB4-induced Ca++-independent release was significant (P < .05) compared to controls.
