Immunofluorescence of GMRβ N-glycosylation mutants in COS cells.

The COS cells grown on chamber slides were transfected with plasmids encoding wild-type or mutated GMRβ subunits and examined by immunofluorescence staining with a rabbit antihuman GMRβ subunit polyclonal antibody. (A) Wild type GMRβ; (B) GMRβ-Ala58; (C) GMRβ-Ala191; (D) GMRβ-Ala 346; (E) GMRβ-Ala58/191/346; (F) GMRβ-Asp58; (G) GMRβ-Asp191; (H) GMRβ-Asp346; (I) GMRβ-Asp58/191/346; (J) mock-transfectant.