American Society of Hematology

Fig. 5.

$Fig. 5. Competitive inhibition of the binding to FVIII between natural anti-FVIII IgG from IVIg and FVIII inhibitors from patients. / Radiolabeled anti-FVIII IgG alloantibody of a high-responder hemophiliac (A) and anti-FVIII IgG autoantibody of an autoimmune patient (B) (3 μg/mL) were incubated with increasing concentrations of anti-FVIII IgG from IVIg (closed squares), unfractionated IVIg (open squares), and IVIg depleted of anti-FVIII antibodies (open triangles) for 4 hours at 37°C. The percentage of inhibition was calculated as described in “Patients and methods.”$

Competitive inhibition of the binding to FVIII between natural anti-FVIII IgG from IVIg and FVIII inhibitors from patients.

Radiolabeled anti-FVIII IgG alloantibody of a high-responder hemophiliac (A) and anti-FVIII IgG autoantibody of an autoimmune patient (B) (3 μg/mL) were incubated with increasing concentrations of anti-FVIII IgG from IVIg (closed squares), unfractionated IVIg (open squares), and IVIg depleted of anti-FVIII antibodies (open triangles) for 4 hours at 37°C. The percentage of inhibition was calculated as described in “Patients and methods.”

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