Minibulk HPC erythroid cultures.

(A) F cells from minibulk HPC erythroid cultures supplemented or not with KL (100 ng/mL) ± TSA (10 ng/mL) ± NaB (0.5 mmol/L). F cells were evaluated by indirect immunofluorescence using an antihuman HbF mAb as a primary antibody and an FITC-conjugated goat antimouse IgG as a secondary antibody. Original magnification × 630. Representative results are shown. (B) Globin chain HPLC scans from mature erythroblasts obtained in minibulk HPC erythroid cultures supplemented or not with KL, TSA, NaB, or combinations thereof (representative results).