Formation of 5′HS 4 and minor sites 5′HS 4.1 and 5′HS 4.2 in mice with single-site deletions assayed by HpaI digestion and probed with probe 3.

The map appearing in (A) is appropriate for all parts of this figure, and is explained in the legend describing (A). (A) HS formation in wild-type mice with a restriction map below the film showing the expected sizes for the HS and parent band. M denotes the marker lane; the DNaseI concentration is 0 in the first lane and increases as shown by the shaded triangle. (B) Similar to (A), with the assay done on mice with 5′HS 1 deleted. (C) Similar to (A), with the assay done on mice with 5′HS 2 deleted. (D) Similar to A, with the assay done on mice with 5′HS 3 deleted.