Formation of 5′HS 1, 5′HS 2, and 5′HS 3 in mice with homozygous single-site deletions assayed by HpaI digestion and probe 1.

(A) HS formation in wild-type mice, showing the expected sites and the parent band. M denotes the marker lane; the DNaseI concentration is 0 in the first lane and increases as shown by the shaded triangle. Below the autoradiograph is a restriction map of expected sizes for the HS and parent band. (B) Similar to (A), with the assays done on mice with 5′HS 1 deleted and the site of the deletion shown on the gel and in the map below. (C) Assay done on mice with 5′HS 2 deleted, with the map below. (D) Assay done on mice with 5′HS 3 deleted, with the map below. (E) Assay done on mice with 5′HS 4 deleted. The map is identical to that in (A). (F) Assay done on mice with 5′HS 5/6 deleted. The map is identical to that in (A).