Fig. 5.
![Fig. 5. Binding of PfEMP1 domains to heparin and heparin fragments. / (A) [3H] Heparin binding to 4 μg of DBL-1 (—•—), CIDR (—♦—), and DBL-4 (—○—) in an in-solution assay was performed with increasing concentrations of [3H] heparin and protein together with bound polysaccharide was recovered by membrane filtration as described in “Materials and methods.” (B) Size defined [3H] heparin fragments were allowed to bind to the proteins under the same conditions as in A: DBL-1 (—•—), CIDR (—♦—), and DBL-4 (—○—).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/11/10.1182_blood.v95.11.3594/5/bloo01121005x.jpeg?Expires=1723508892&Signature=RHXCUKUv4H2B8KZEcBt7fPp25kuPy9-wfHSzYskkTsaDHoF2vVESL3EIqUrolFucmxc1RLxWJ2K95ocm3ZXADvKZpkfigFwCBEfE0ZVJ8cmSGszJXbYa2GALWjdFxxB19aHZYx2akiKn5KpiCLQl1a75HJOIL4UoFPKlX~N5pQc0Pcr6eNxN3Iem1nbBbSnl1Awx9ulRHw-FkBW7B04G87HwDcrilflv249ZpdRcMKqPPRen970q6joeZtYgm3OkYHovYxRaZxPVAB413SVpTKbWg6ribKTtDoH4G1zIdR9vWuO~MHU0zu1Sd6d5sZBB-LfjFDXIX38OIm0iv2dFYQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Binding of PfEMP1 domains to heparin and heparin fragments.
(A) [3H] Heparin binding to 4 μg of DBL-1 (—•—), CIDR (—♦—), and DBL-4 (—○—) in an in-solution assay was performed with increasing concentrations of [3H] heparin and protein together with bound polysaccharide was recovered by membrane filtration as described in “Materials and methods.” (B) Size defined [3H] heparin fragments were allowed to bind to the proteins under the same conditions as in A: DBL-1 (—•—), CIDR (—♦—), and DBL-4 (—○—).
