American Society of Hematology

Fig. 4.

$Fig. 4. Affinity purification of infected erythrocyte membrane proteins by heparin–Sepharose. / Cell extracts of surface 125I-labeled FCR3S1.2 pRBC were incubated with Sepharose-linked heparin and the bound fraction analyzed by SDS-PAGE as described in “Materials and methods.” The specificity of binding was assessed by competition with the indicated amounts of soluble heparin, HS and CSA.$

Affinity purification of infected erythrocyte membrane proteins by heparin–Sepharose.

Cell extracts of surface ¹²⁵I-labeled FCR3S1.2 pRBC were incubated with Sepharose-linked heparin and the bound fraction analyzed by SDS-PAGE as described in “Materials and methods.” The specificity of binding was assessed by competition with the indicated amounts of soluble heparin, HS and CSA.

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