Bis derivatives facilitate apoptosis induced by anti-CD95 mAb.

DC were incubated in medium in the presence of anti-CD95 mAb (0.5 μg/mL) with or without Bis I, II, III, IV, or VIII (20 μmol/L). After 18 hours, cells were fixed, DNA was stained with PI, and the percentage of hypodiploid nuclei (subG1 peak) was analyzed with use of flow cytometry. Data are representative results from 3 experiments done on samples from different blood donors.