FACS analysis of BM at early time points after transplantation.

Sheep that had been transplanted in utero with human HSC alone or in combination with autologous stromal cells were killed at early time points of 3, 6, and 9 weeks after transplant and their long bones collected. Single cell suspension were then obtained as detailed in “Materials and methods,” and FACS analysis for human-specific cell markers was performed to evaluate whether stromal cells could enhance the levels of engraftment of donor human cells within the animals transplanted in utero. Values represent the mean ± 1 SEM.