HCQ does not alter MAP kinase activation.

Jurkat T cells were treated with varying concentrations of HCQ for 24 hours, stimulated with C305 for 0 to 5 minutes, and then lysed in NP-40 detergent. Proteins from whole-cell lysates were subjected to 12% SDS-PAGE and were transferred to nitrocellulose, and membranes were immunoblotted with anti-ERK-2. Immunoreactive bands were visualized by enhanced chemiluminescence. Representative data from more than 3 similar experiments are shown.