Fig. 4.
![Fig. 4. HCQ does not inhibit TCR-induced inositol phosphate production. / Jurkat T cells were treated with varying concentrations of HCQ for 24 hours and then loaded with [3H] myoinositol for 3 hours. Cells were left untreated (none) or were stimulated with C305 for 15 minutes, and were then lysed in chloroform. Soluble inositol phosphates were extracted by anion exchange and quantitated by liquid scintillation counting. Mean data from 3 separate experiments are expressed as stimulation indices: (cpm stimulated/cpm unstimulated) ± SD.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/11/10.1182_blood.v95.11.3460/6/bloo01126004x.jpeg?Expires=1724226866&Signature=CNu4jYP~QXtlyry-7zImos5aL4dwiybLJVREssoOb26r7-6YRihFfH5SMBVkO4livujDUfnRbvl8Wya6hHVPBsxHlaIlvx7t1nzPYD6JRUxwA7Vn3cGpj2wL0ksNrl6KlHyrLEhcZvYgsrel5lSwbyO1Jjx71FMa2p-KqqPu0nlx9LT7RP7UOAdy9J7FKv~wdv2WiddKt6lcUzKRxSFjDeJZbdYbzmQ25uykgAap6j-uR8K1CLXDF4KDLzcY44SHdJEioeGA0rfuqL4K2WiwLIrklnjrXDzswkbheVUh48QTMmlhd2bqWd~dF9fCuHX6OpXPoD-oiufXsupmhi1DhQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
HCQ does not inhibit TCR-induced inositol phosphate production.
Jurkat T cells were treated with varying concentrations of HCQ for 24 hours and then loaded with [3H] myoinositol for 3 hours. Cells were left untreated (none) or were stimulated with C305 for 15 minutes, and were then lysed in chloroform. Soluble inositol phosphates were extracted by anion exchange and quantitated by liquid scintillation counting. Mean data from 3 separate experiments are expressed as stimulation indices: (cpm stimulated/cpm unstimulated) ± SD.
