Fig. 3.
HCQ does not alter TCR-induced PLCγ1 tyrosine phosphorylation.
Jurkat T cells were treated with varying concentrations of HCQ for 24 hours, stimulated with C305 for 15 minutes, and then lysed in NP-40 detergent. PLCγ1 was immunoprecipitated from 5 × 107 cell equivalents, and immunoprecipitates were blotted with either antiphosphotyrosine (upper panel) or anti-PLCγ1 (lower panel), and immunoreactive bands were visualized by enhanced chemiluminescence. Representative data from more than 3 similar experiments are shown.
