Fig. 2.
HCQ does not inhibit TCR-mediated inductive protein tyrosine phosphorylation.
Jurkat T cells (1 × 106) were treated with varying concentrations of HCQ for 24 hours, stimulated with C305 for 5 minutes, and then lysed in NP-40 detergent. Proteins from whole-cell lysates were separated by 10% SDS-PAGE, transferred to nitrocellulose, and immunoblotted with antiphosphotyrosine antibody (4G10). Immunoreactive bands were visualized by enhanced chemiluminescence. Representative data from more than 3 similar experiments are shown. A more extended kinetic analysis of protein tyrosine phosphorylation in untreated and HCQ-treated cell populations gave similar results (data not shown).
