Transcription rates of genes encoding gp91-phox, and p47-phox in CGD patient's and normal B-cell lines.

Upper panel: Representative nuclear run-on assay showing transcription rates of genes encoding gp91-phox (CYBB), and p47-phox (NCF1) in CGD and normal B cell lines. Lower panel: Graph representing compiled data from 3 experiments; columns indicate means and error bars show SDs. EBV-transformed B cells were cultured under control conditions or with IFN-γ (100 U/mL) and TNF-α (1000 U/mL) for 7 days. The negative control lanes contain pBluescript plasmid alone. Genes encoding α-tubulin and β-actin were included as transcriptionally constitutive controls.