Northern blot of mRNA transcripts encoding gp91-phox and p47-phox.

Each lane contains 10 μg total RNA from IFN-γ–differentiated HL-60 cells, normal, and CGD B-cell lines, or HeLa cells, as indicated above. The autoradiograph shows a representative blot successively probed with³²P-labeled cDNAs encoding gp91-phox, p47-phox, and (as a control for equal loading of lanes) 18S rRNA, as indicated in the left margin. The EBV-transformed B-cell lines were cultured in control conditions or in the presence of IFN-γ (100 U/mL), TNF-α (1000 U/mL), alone or in combination for 7 days, as indicated in the top margin.