Mutation of the EGR-1 consensus sequence in theflt-1 promoter dramatically decreases its transcriptional activity after mechanical denudation.

(A) HUVEC were transiently transfected with the pFlt-1–Luc plasmid or the mutated version (see mutation in Figure 7A) pmutFlt-1–Luc. Cell monolayers were mechanically denuded, and luciferase activity was measured after 8 hours. (B) HUVEC were transiently cotransfected with the luciferase reporter plasmids pFlt-1–Luc or pmut-Flt-1–Luc and the EGR-1 expression vector pBXEGR-1 or the empty vector pBX. Luciferase activity was measured 24 hours after transfection. Number of experiments, normalization, and statistics are similar to those in Figure 3.