Mutation of the EGR-1 binding site at −24 to −16 bp in the flt-1 promoter.

(A) Schematic representation of the mutation of the Egr-1 binding site; mutated base pairs are represented in bold characters. (B) Electrophoretic mobility shift assays were performed using conditions similar to those in Figure 5A but competing with the indicated molar excess of the mutated probe (mut) or the wild-type probe (wt), both represented in (A). C, nuclear extract from control cells. Similar results were obtained in 2 independent experiments.