Induction of Egr-1 expression in endothelial cells after mechanical denudation.

HUVEC monolayers were denuded and formaldehyde fixed after 0 (A, C, E) or 1 hour (B, D, F). (A, B) Cells were incubated with a rabbit-specific IgG against Egr-1, (C, D) with a rabbit specific IgG against Sp-1 (red), and (E, F) with the secondary antibody alone (same as Figure 1). All preparations were stained with fluorescein-conjugated phalloidin to localize the cells at the edge of the denuded area.