Fig. 2.
Endothelial denudation increases Flt-1 mRNA level.
HUVEC monolayers were mechanically denuded, and total RNA was extracted at the indicated times. (A) Northern blot analysis was performed with 20 μg total RNA per lane. Nylon filters were hybridized to32P-labeled flt-1 or β-actin probe. (B) Values represented in the graphic are the data obtained from densitometry of the signals corresponding to the flt-1 mRNA normalizing to the corresponding β-actin levels. Similar results were obtained in an independent experiment.
