mRNA expression studies with RT-PCR–amplified fragments of FVII mRNA expressed in BHK cells cotransfected with pIVS7H5 and pIVS7 + 5A (internal control).

The following pIVS7 + 5A/pIVS7H5 DNA concentration ratios were used in lanes 1-4: 1:1, 1:0.3, 1:0.15, and 1:0.1, respectively.