Phase-contrast microscopy of human GMVECs.

Magnification × 50, except for (B) (magnification × 100). (A) Represents control GMVECs. (B) Shows the result of GMVECs incubated with PMNs for 24 hours. No change of morphology was observed. When cells were incubated with PMNs loaded with VT-1, cell death was observed after 24-hour incubation (C). (D) Represents control TNF-α–treated GMVECs. The effect of PMNs loaded with VT-1 was also studied on TNF-α–treated GMVECs (E) and compared with the effect of VT-1 on TNF-α–treated GMVECs (F). In both conditions, equal amounts of cell death were observed.