Fig. 3.
Fig. 3. PMNs of 2 different donors were used to determine binding of 125I-VT-1 to PMNs. / (A) PMNs were incubated with increasing concentrations of125I-VT-1 (0.3 to 70 nmol/L) at 4°C for 3 hours (▴). Nonspecific binding (•) was determined in the presence of 25-fold excess of unlabeled VT-1. (B) Shows the result of Scatchard plot analysis.

PMNs of 2 different donors were used to determine binding of 125I-VT-1 to PMNs.

(A) PMNs were incubated with increasing concentrations of125I-VT-1 (0.3 to 70 nmol/L) at 4°C for 3 hours (▴). Nonspecific binding (•) was determined in the presence of 25-fold excess of unlabeled VT-1. (B) Shows the result of Scatchard plot analysis.

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