American Society of Hematology

Fig. 3.

$Fig. 3. Analysis of fractions from Ni-column chromatography. / Three fractions of wash buffer and 5 fractions of 250 mmol/L imidazole on 0.5 mol/L NaCl fractions of eluate were collected. (A) 10 μL were loaded onto a 10% SDS-PAGE gel and stained with Coomassie blue. (B) 10 μL was loaded on SDS-PAGE, transferred, and detected with anti-PDE3A polyclonal antibody as described in “Materials and methods.”$

Analysis of fractions from Ni-column chromatography.

Three fractions of wash buffer and 5 fractions of 250 mmol/L imidazole on 0.5 mol/L NaCl fractions of eluate were collected. (A) 10 μL were loaded onto a 10% SDS-PAGE gel and stained with Coomassie blue. (B) 10 μL was loaded on SDS-PAGE, transferred, and detected with anti-PDE3A polyclonal antibody as described in “Materials and methods.”

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