Time course of expression of recombinant PDE3A in Sf9 cells.

(A) At each time point, cells were collected, counted, and lysed, and the enzymatic activity and total protein were measured as described in “Materials and methods.” The left lane contains the molecular weight standards. (B) At each time point, 10 μg protein was applied to a 10% SDS gel, transferred electrophoretically, and analyzed by Western blotting as described in “Materials and methods.”