Labeling of Reed-Sternberg cells with anti–MAGE-A4 MAb 57B in nodular sclerosing lymph nodes.

Patients include HD3 (A and B) and HD4 (C) at low (A), high (B) and intermediate (C) magnification. (B) and (C) correspond to MAGE-A4expression in HD3. Formalin-fixed sections were deparaffinized, heated in a citrate buffer, and incubated with MAb 57B. The antibody was detected with biotinylated secondary antibodies and avidin-coupled alkaline phosphatase. The chromogenic substrate was New Fuschin, and hematoxylin was used to counterstain the tissue.