Fig. 1.
Fig. 1. In vitro crystal formation in 8A4 cells. / (A) Light microscopy of living cells (original magnification ×400). (B) Immunofluorescence on acetone/ethanol-fixed cells stained with fluorescein-labeled anti-IgG (original magnification ×400). (C and D) Confocal fluorescence microscopy of 8A4 cells: hybridoma cell stained with antimouse IgG-Cy3 conjugate. Rod-like intracellular crystals display strong signals. Intensities are coded by pseudocolors (C) or brightness (D). Scale bar: 2 μm.

In vitro crystal formation in 8A4 cells.

(A) Light microscopy of living cells (original magnification ×400). (B) Immunofluorescence on acetone/ethanol-fixed cells stained with fluorescein-labeled anti-IgG (original magnification ×400). (C and D) Confocal fluorescence microscopy of 8A4 cells: hybridoma cell stained with antimouse IgG-Cy3 conjugate. Rod-like intracellular crystals display strong signals. Intensities are coded by pseudocolors (C) or brightness (D). Scale bar: 2 μm.

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