Expression of mRNA of Mgf, Flt3l, and Il7in the osteopetrotic mice.

Total RNAs were prepared from bone marrow, spleens, or livers of 8-week-old osteopetrotic mice. Different dilutions (1:1, 1:8, 1:64) of cDNA were subjected to PCR amplification specific for Mgf, Flt3l, Il7, and GAPD transcripts. The products were electrophoresed, blotted, and probed to detect messages for each corresponding gene.