Genomic DNA fragments of FVII amplified by PCR were digested with HgiC I endonuclease and electrophoresed on a 12% polyacriminate gel.

Another HgiC I endonuclease restriction site was generated by a T → G mutation at codon 329 of the FVII gene, leading to the occurrence of 3 fragments (207, 82, and 69 bp) in the patient, instead of 2 fragments (207 and 151 bp) in the healthy subject. A heterozygous restriction pattern was shown in the patient's son. M indicates DNA markers (πx174/Hae III); N, normal subject; P, patient; S, patient's son.