Assessment of alloengraftment in central (BM, thymus) and peripheral (spleen) lymphohematopoietic compartments. BM (A), thymus (B), and spleen (C) was obtained from 4- to 6-month-old chimeras (n = 5 to 6 mice/group) and analyzed for the presence of donor cells. In BM and spleen, the number of donor CD4⁺, CD8⁺, CD19⁺ B cells, donor Mac1⁺(Mac1^b), and host Mac1⁺ (Mac1^d) cells were quantified. The thymus was analyzed for T-cell differentiation consisting of immature CD4⁻8⁻, intermediate CD4⁺8⁺, and mature CD4⁺8⁻ or CD4⁻8⁺ cells. The absolute number of cells is shown on the y-axis. One standard error of the mean values for absolute cell number are designated by bars. *Significant differences between IUT TCD recipients and other groups. #Significant differences between the indicated groups and the non-BMT B6 control mice.