Fig. 9.
![Fig. 9. Detection of gene expression of IL-3 and GM-CSF in GFP-positive and GFP-negative bone marrow cells from Bcr-Abl–BMT mice. Bone marrow cells from Bcr-Abl–BMT mice (BMT8.40 [lanes 1 through 4] and BMT8.41 [lanes 5 through 8]) were isolated and treated with ACK to lyse RBC. GFP-positive and GFP-negative bone marrow cells were sorted by flow cytometry and their RNA was extracted as described in Materials and Methods. The RT-PCR was performed and analyzed as in Fig8. The RT-PCR products of IL-3, GM-CSF, GFP, and GAPDH are indicated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/10/10.1182_blood.v92.10.3829/4/blod42214009w.jpeg?Expires=1724866252&Signature=UWd51DVDe95GeRJ6G0rfbTNmC8NhOr6dC2rXQCYeFL-MvzkT1y1T5ps0QtcBDkicrZsK~gjQJVZ3BW9pemavEjMzi9FGsD35~fK-8KQPJGQHTTqpOfGr6oH2usEPbtYbtU1EHKwEJDOxT4ApKXRf4SK~s8CBsnNTkn9nzXnhN4Y1vEdmztKdEzgo6gtAmr~ZFy~V~n8kZSc-zvZYk~6uIGVsUIFyCKiQ0W81E85mMx8bQWXzu4ryyahc4dx4bWoPx3PcDRX64-tw9VxjRDjIzCtA-ZlvQ-LiQ0X9vx5l3imUPjkYmzaFZEpCkMy846PGbOHouWA3x7ormVX1Dfjh~A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Detection of gene expression of IL-3 and GM-CSF in GFP-positive and GFP-negative bone marrow cells from Bcr-Abl–BMT mice. Bone marrow cells from Bcr-Abl–BMT mice (BMT8.40 [lanes 1 through 4] and BMT8.41 [lanes 5 through 8]) were isolated and treated with ACK to lyse RBC. GFP-positive and GFP-negative bone marrow cells were sorted by flow cytometry and their RNA was extracted as described in Materials and Methods. The RT-PCR was performed and analyzed as in Fig8. The RT-PCR products of IL-3, GM-CSF, GFP, and GAPDH are indicated.
