Fig. 8.
![Fig. 8. Detection of gene expression of cytokines in bone marrow of transplanted mice by RT-PCR. Bone marrow cells from Bcr-Abl–BMT mice (BMT5.1 [lanes 1 and 2], BMT5.4 [lanes 3 and 4], and BMT5.6 [lanes 5 and 6]) and a vector-BMT mouse (BMT5.35 [lanes 7 and 8]) was collected, and RNA was extracted as described in Materials and Methods. The RT-PCR products generated with (RT+) or without (RT−) reverse transcriptase were subjected to agarose gel electrophoresis, stained with ethidium bromide, and photographed by Gel Doc 1000 (Bio-Rad, Hercules, CA) with inverse gray scale. The RT-PCR products of IL-3, GM-CSF, and SCF are indicated. GAPDH was used as a control for the quality and quantity of the RNA samples and the RT-PCR reactions.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/10/10.1182_blood.v92.10.3829/4/blod42214008w.jpeg?Expires=1724865970&Signature=01l6FZhBALaJOV512Z~zjHv9r-aXWq4E46dg8M2Wwt8LJ3KtZCGlHk78CPNF8-QaFzLc~TXK77u-wH538qtsjhyMjyfGdrwaguADYFoZ4KJDQbPhaL7m7wCI800ydHNvF4-Z1AH1joKcslfZ1sr4iJAMNki4myEFp7jE8AiSLyjhHHPZhv7KEhg0mtTNc7dMAqUq2JxeRifNIaMiwEbVTW1YjoO08tERUkWg2s85vs8AOG-kbj69fERwQhArxXuIyUn-sGyDrKIVoheDdHhisl4OMr0fk3qXnvdIh5hYngGapQsG1v19ThMs5L1t~eQjYHO5YKKcUIaJAwbGIi3ryg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Detection of gene expression of cytokines in bone marrow of transplanted mice by RT-PCR. Bone marrow cells from Bcr-Abl–BMT mice (BMT5.1 [lanes 1 and 2], BMT5.4 [lanes 3 and 4], and BMT5.6 [lanes 5 and 6]) and a vector-BMT mouse (BMT5.35 [lanes 7 and 8]) was collected, and RNA was extracted as described in Materials and Methods. The RT-PCR products generated with (RT+) or without (RT−) reverse transcriptase were subjected to agarose gel electrophoresis, stained with ethidium bromide, and photographed by Gel Doc 1000 (Bio-Rad, Hercules, CA) with inverse gray scale. The RT-PCR products of IL-3, GM-CSF, and SCF are indicated. GAPDH was used as a control for the quality and quantity of the RNA samples and the RT-PCR reactions.
