Inhibition of nucleotide-induced currents in HEK293 cells stably transfected with hP2X7 by MoAb. (A) Comparison of effects on various P2X receptors. In each panel, an initial application of an appropriate purinergic agonist (see Materials and Methods) was made to HEK293 cells stably transfected with one of five P2X receptors (line 1). Cells were incubated with the MoAb (1.15 μg/mL) for 10 minutes and a second application of the same agonist was made in the presence of the MoAb (line 2). A final application of the agonist was made after 10 minutes of washing (except for hP2X7, which was for 30 minutes) in the absence of the MoAb (line 3). (B) Concentration-dependent inhibition of hP2X7 channel function by the MoAb. Points represent the percentage of maximal current after incubating cells for 10 minutes in varying concentrations of the MoAb.
