Fig. 2.
Detection of P2X7 receptors by flow cytometry on human blood-derived monocytes cultured for (A) 1 day, (B) 2 days, or (C) 3 days in complete RPMI medium (monocytes) or with the addition of LPS (10 μg/mL) or γ-IFN (10 ng/mL). Antibodies were used as in Fig 1, with the control showing incubation of monocytes with an IgG2b isotype control. Monocytes were derived from PBMC by rosetting and FACS using forward and side scatter.
