Fig. 1.
Characterization of hP2X7 receptor MoAb by flow cytometry with HEK293 cells stably transformed with (A) hP2X1, (B) hP2X4, or (C) hP2X7. Cells detached with PBS plus 1 mmol/L EDTA were incubated on ice with 15 μg/mL purified antibody for 30 minutes. The MoAb (bold line) was detected with an FITC-labeled sheep antimouse F(ab′)2fragment. An IgG2b antibody (thin line) was used as an isotype control.
