Eosinophils tether and roll on VCAM-1–transfected L cells. Confluent monolayers of nontransfected and VCAM-1–transfected L cells were washed once with HBSS and then placed in flow assembly. Eosinophils (5 × 10⁵/mL) were perfused over these cells at the specified wall shear stresses. (A) Accumulation, (B) rolling, and adherence were determined as described in Materials and Methods. In some experiments, eosinophils were pretreated for 10 minutes at 37°C with 2 μg/mL of H2/1, an anti–4-integrin MoAb, before perfusion over VCAM-1–transfected cells. The data represent the mean and SEM of at least three independent experiments.