American Society of Hematology

Fig. 7.

$Fig. 7. p62dok, p21cip1, and p27kip1 expression in proliferating and differentiating erythroid cells. SCF/Epo-dependent erythroid progenitor cells at different stages of differentiation were analyzed for p62dok, p21cip1, and p27kip1expression by Western blotting. Band 3 expression is shown to demonstrate efficient differentiation. Lane 1, undifferentiated progenitor cells at day 9 of culture. Lanes 2 through 4, differentiated cells at day 3 of differentiation following fractionation by Percoll density centrifugation. Samples were normalized for equal protein loading per lane (30 μg). The proportion of proerythroblasts, polychromatic and orthochromatic erythroblasts, and erythrocytes is indicated.$

p62^dok, p21^cip1, and p27^kip1 expression in proliferating and differentiating erythroid cells. SCF/Epo-dependent erythroid progenitor cells at different stages of differentiation were analyzed for p62^dok, p21^cip1, and p27^kip1expression by Western blotting. Band 3 expression is shown to demonstrate efficient differentiation. Lane 1, undifferentiated progenitor cells at day 9 of culture. Lanes 2 through 4, differentiated cells at day 3 of differentiation following fractionation by Percoll density centrifugation. Samples were normalized for equal protein loading per lane (30 μg). The proportion of proerythroblasts, polychromatic and orthochromatic erythroblasts, and erythrocytes is indicated.

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