Butyrate-induced changes in histone H3 acetylation in sickle cell disease.

A: Acetylation of histone H3 in BFU-E or CFU-GM derived cells from a patient with sickle cell disease. The bar graph shows the results of ChIP analysis of the promoters of the ε-, γ-, and β-globin genes expressed as relative units (RU).

B: mRNA levels of different globins measured by real-time PCR and expressed as fold change between control BFU-E cells and butyrate exposed BFU-E cells.

C: mRNA levels of different globins measured by real-time PCR and expressed as ratios in control BFU-E cells and butyrate exposed BFU-E cells.