Restoration of NKG2D function. (A) After 3 days of coculture with RMA (□) or RMA-H60 cells (•), residual tumor cells were removed and NK cells were cultured for an additional 18 hours with IL-2. The cytolytic activity of the NK cells was determined toward RMA-H60 targets. The graph depicts the mean (±SD) of triplicate determinations at various E/T ratios. The results shown are representative of 5 independent experiments performed. (B) Immunoblots of total cell lysates from “recovered” NK cells using the indicated Abs.