BCG is neither associated with DCs nor with macrophages in the dermis. (A) From 4 to 72 hours after BCG-egfp inoculation, skin explant cells were stained with CD11c and analyzed by flow cytometry. (B) Ear cryosections from BCG-egfp and mock-injected mice were immunostained with antibodies directed against CD11c and mMGL, a receptor largely distributed among dermal phagocytes. Colocalization of BCG (green) with these cell populations (red) could not be observed. After labeling with Texas red (CD11c) or Alexa 594 (mMGL), slides mounted in Fluoromount were analyzed under a Zeiss Axioskop fluorescence microscope with an Achroplan 20 ×/0.45 NA objective. Images were acquired with a Leica DC300F camera and Leica Qwin software. Images were then processed with Adobe Photoshop (Adobe Systems, San Jose, CA).