RT-PCR products of CD95 and CD95-del6 mRNA. (A) RT-PCR before (–) and after (+) activation (5 days as in Figure 2) of PBMCs (T cells) and of EBV-transformed B cells (B cells, n = 5). P, patient; C, healthy donor; primers: CF2, CR2. (B) Real-time TaqMan RT-PCR of the splice product containing exon 6 (—) and of the alternatively spliced mRNA without exon 6 (- - -), both from PBMCs. Primers and probes: TF1, P1,TR1; TF2, P2, TR2; abscissa: days of activation; ordinate: reciprocal cycle threshold. The same experiment was performed with EBV-transformed B cells (same result as on day 5, n = 3, not shown).