Figure 2. Apoptosis of lymphocytes from the patient and the other family members. (A) PBMCs were activated for 5 days (2 days PHA, 3 days IL2) and incubated in the absence and presence of agonistic mAb CH-11 (2.5 μg/mL) for another 24 hours. Ordinate: percent PI- and annexin-positive cells; induced minus spontaneous apoptosis is given. (B) Proliferating EBV-transformed B cells from the patient were incubated with mAb CH-11 at different concentrations. Five experiments with 2.5 μg/mL and 25 μg/mL mAb CH-11 each. Error bars indicate standard deviation. • indicates cells from a healthy donor; ♦, cells from the patient. Induced minus spontaneous apoptosis is given. Ordinate as in A.
Figure 2.

Apoptosis of lymphocytes from the patient and the other family members. (A) PBMCs were activated for 5 days (2 days PHA, 3 days IL2) and incubated in the absence and presence of agonistic mAb CH-11 (2.5 μg/mL) for another 24 hours. Ordinate: percent PI- and annexin-positive cells; induced minus spontaneous apoptosis is given. (B) Proliferating EBV-transformed B cells from the patient were incubated with mAb CH-11 at different concentrations. Five experiments with 2.5 μg/mL and 25 μg/mL mAb CH-11 each. Error bars indicate standard deviation. • indicates cells from a healthy donor; ♦, cells from the patient. Induced minus spontaneous apoptosis is given. Ordinate as in A.

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