Analysis of human hematopoietic cells in NOD/SCID/IL2rγ^nullrecipients. (A) In the scatter gates for nucleated cells, anti-hCD45 and anti-mCD45 antibodies (Abs) reacted exclusively with human and murine leukocytes, respectively. In the recipient blood, the majority of nucleated cells were human leukocytes (top row). High levels of engraftment by hCD33⁺ myelomonocytic cells, hCD19⁺ B cells, and hCD3⁺ T cells were achieved in peripheral blood of recipient mice given transplants of Lin^–hCD34⁺ CB cells (bottom row). (B) Analysis of circulating erythrocytes (top row) or platelets (bottom row) in a NOD/SCID/IL2rγ^null recipient. In the blood, Ter119⁺ murine erythrocytes as well as hGPA⁺ human erythrocytes were detected. mCD41a⁺ murine platelets were also reconstituted. (C) Multilineage engraftment of human cells in the NOD/SCID/IL2rγ^null murine bone marrow. hCD33⁺ myelomonocytic cells, hCD19⁺ B cells, and hCD3⁺ T cells were present. hGPA⁺ erythroid cells and hCD41a⁺ megakaryocytes were also seen in the nucleated cell gate of the bone marrow. (D, left) HLA-DR⁺hCD11c⁺ dendritic cells were detected in the spleen by a flow cytometric analysis. (Right) Immunohistochemical staining of CD11c in the spleen. CD11c⁺ cells displayed dendritic cell morphology.