Content of PSGL-1, TF, and CD45 in monocytes and their microvesicles (MVs). THP-1 cells or blood monocytes were stimulated with LPS; microvesiculation was induced with calcium ionophore. Cells were separated from their shed MVs by sedimenting the cells at 5000g for 5 minutes. The entire cell pellet and MV suspension were resuspended and diluted to the same extent, and lysed in SDS sample buffer. Equal volumes of the lysates were subjected to SDS-PAGE and analyzed by Western blot. (A) PSGL-1 and TF content in THP-1 cells and their shed MVs. (B) TF and CD45 content in monocytes, THP-1 cells, their respective MVs, and in MVs isolated from fresh plasma. (C) PSGL-1 and CD45 content in monocytes and their shed MVs. (D) Densitometric analysis of the MV/cell ratio of PSGL-1, TF, and CD45. These data are represented as the mean value for the MV/cell ratio in 6 different experiments plus or minus SD. *P < .003 CD45 versus PSGL-1 and versus TF.