Inducible expressions of Aurora-B kinase in megakaryocytes. Primary bone marrow cell mRNA was isolated from double-transgenic mice (PF4-tTA-VP16/CMV–Aurora-B/β-gal no. 41/42) treated with and without doxycycline (Tet-on or Tet-off) and reverse transcribed to generate cDNA. PCR was subsequently conducted using primer sets specifically designed to amplify FLAG-tagged rat Aurora-B kinase. GAPDH was amplified to show that the RT-PCR was successfully conducted in all samples. GAPDH-specific primers and PCR cycles were performed, as indicated in “Materials and methods.”