Induction of CD4⁺CD25^bright regulatory T cells in rats undergoing BMT. (A) Representative FACS blots of DA rats without BMT and with BMT with DA or ACI BM. (B) CD4⁺CD25^bright cells in LNs of DA rats without BMT and with BMT (DA rats without BMT [n = 8], DA rats after BMT with BM from DA rats [n = 6] or ACI [n = 6] rats). Numbers in graphs represent percentages of gated lymphocytes in each quadrant. There were differences between the groups receiving and not receiving transplants (^*P < .01, ANOVA). (C) Foxp3 expression in spleen cells from DA rats without BMT (n = 7) and with BMT with syngeneic BM (n = 7). There were differences between the groups receiving and not receiving transplants (^*P = .05, t test). (D) Transfer of CD4⁺CD25⁺ T cells results in protection from MOG-induced EAE in DA rats as compared with rats transferred with CD4⁺ T cells depleted of CD25⁺ cells (n = 7 each group, P < .001, t test). Repeated experiments showed similar results (n = 3 each group).