Chimerism and engraftment. (A) Chimerism was determined in DA rats grafted with ACI BM (n = 6) and ACI rats grafted with DA BM (n = 8) on day 107 after BMT from blood by a microsatellite as described in “Materials and methods.” (B) EAE was induced with MBP 63-85 in the LEW/eGFP-LEW bone marrow chimeras on day 54 after BMT. The LEW/eGFP-LEW chimeric rats with EAE (n = 5) showed a similar disease course compared with a LEW rat control group not undergoing transplantation (n = 5). (C) eGFP-expressing cells in LNs in naive eGFP-expressing LEW rats (pool of 5) and LEW rats receiving transplants with BM from eGFP transgenic rats and subsequently induced with EAE (n = 5). (D) Example of the numbers of eGFP-expressing CNS-infiltrating cells eluted from the CNS of a LEW rat with MBP-induced EAE that had undergone transplantation with BM from an eGFP transgenic LEW rat. Numbers in graph represent percentages of gated lymphocytes in each quadrant.