Expression of Wnts induces RhoA/PKCμ association. (A) Cell lysates from Wnt-transfected clones were incubated with anti-RhoA antibody. Immunoprecipitates (IP) were subjected to 8% SDS-PAGE, transferred to membranes, and blotted with anti-PKCμ antibody. IB indicates immunoblot. (B) Cells starved in serum-free medium and then pretreated with indicated concentrations of Rho-kinase inhibitor Y27632 (2.5-10 μM) were untreated or stimulated with Wnt-3a for 10 minutes. Cell lysates were subjected to SDS-PAGE and blotted with anti-p-PKCμ.