ICAM-2 is involved in endothelial-cell migration. WT-MCEC-SV and IC2-MCEC-SV cells were used in a wound-healing assay. Confluent monolayers were scratch-wounded and incubated for up to 48 hours. (A) Photographs of wounded monolayers taken at 1 and 23.5 hours. The vertical lines indicate the wound edge and the migration front (dotted line). The horizontal arrows indicate the migration distance. Scale bar equals 50 μm. Images were acquired as in Figure 1A. (B) Migration distance, measured at 14, 18, 22.5, and 23.5 hours. IC2-MCEC-SVs show decreased migration at all time points, compared with WT. Values represent mean ± SD of 8 measurements. *P < .001, unpaired t test.